Journal: Scientific Reports

Article Title: Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair

doi: 10.1038/s41598-017-05699-x

Figure Lengend Snippet: Core/Shell 3D printing by co-axial extrusion. ( A ) Schematic representation of the 3D co-axial handheld printer. ( B ) Schematic representation of the co-axial nozzle. ( C ) Picture of the cartridges dedicated to Core and Shell loading in the printer, with relative magnification of the nozzle during co-axial deposition. ( D ) Representative 3D rendered confocal images of Core/Shell printed sample labeled with fluorescent beads. The Shell (GelMa/HAMa plus LAP 0.1%) is shown in red channel, while the Core (GelMa/HAMa) is shown in green channel. z -stacks were acquired every 10 µm and 3D rendering was performed with NIS elements software using the Alpha - blending algorithm. A Nikon Plan Fluor 10x DIC L N1 NA0.3 objective lens was used. The panel shows the same image representative of 3D rendered of superimposed green and red channels in three different orientations.

Article Snippet: Confocal imaging was performed with NikonA1R confocal microscope using a Nikon Plan Fluor 10x DIC L N1 N.A.

Techniques: Labeling, Software

Journal: Scientific Reports

Article Title: Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair

doi: 10.1038/s41598-017-05699-x

Figure Lengend Snippet: The Core/Shell design allows cells to proliferate. ( A ) Representative 3D rendered confocal images of ADSCs bioprinted samples stained with Calcein-AM (live cells, green channel) at day 1 and day 15 after printing. The Core/Shell samples were generated by labeling with fluorescent beads the Shell (GelMa/HAMa plus LAP 0.1%, cyan channel). z -stacks were acquired every 10 μm and 3D rendering was performed with NIS elements software using the Alpha - blending algorithm. A Nikon Plan Fluor 10x DIC L N1 NA0.3 objective lens was used. Images show representative 3D rendered of superimposed (merge) cyan and green channels of confocal single 2D z -stacks. ( B ) The graph shows the representation of the percentages of the same area occupied by cells (Cells percentage) and Shell (Shell percentage). ( C ) Fluorescence images of 10 μm cryosections obtained from the cryopreserved bioprinted samples. The cyan channel represents the labeled Shell, while cells have been stained with DAPI before imaging. A fluorescent Olympus IX70 inverted microscope with a SPOT Diagnostic RT-Slider camera and SPOT Diagnostic software was used. Olimpus 4X UPlanFL NA0.13, 10X CPlanFL RC NA0.3 and 20X LCPlanFL RC2 NA0.4 objective lenses were used.

Article Snippet: Confocal imaging was performed with NikonA1R confocal microscope using a Nikon Plan Fluor 10x DIC L N1 N.A.

Techniques: Staining, Generated, Labeling, Software, Fluorescence, Imaging, Inverted Microscopy, Diagnostic Assay